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Mouse CD16/CD32 Antibody – Clone 2.4G2 (Anti-FcγRII/III Blocking Antibody)

The Mouse CD16/CD32 Antibody – Clone 2.4G2 is a rat monoclonal antibody widely used to block FcγRII (CD32) and FcγRIII (CD16) receptors in mouse models. By inhibiting Fc-mediated binding of antibodies to these receptors, clone 2.4G2 reduces non-specific background in flow cytometry and improves the specificity of in vivo and in vitro functional assays.

Product Overview

  • Name: Mouse CD16/CD32 Antibody – Clone 2.4G2
  • Clone: 2.4G2
  • Host species: Rat
  • Reactivity: Mouse
  • Target: FcγRII (CD32) and FcγRIII (CD16)
  • Primary function: Fc receptor blocking (anti-CD16/CD32)

Key Features & Benefits

  • Dual FcγR blocker
    Specifically blocks mouse FcγRII (CD32) and FcγRIII (CD16), preventing Fc-mediated binding of other antibodies and immune complexes.
  • Reduces background staining
    Essential for flow cytometry panels using mouse cells, especially when using unconjugated or Fc-containing antibodies, by reducing non-specific Fc receptor–mediated binding.
  • Improves in vivo assay specificity
    Frequently used in in vivo depletion, antibody therapy, and immune modulation studies, to minimize unwanted Fc receptor engagement.
  • Rat anti-mouse monoclonal
    Rat-derived clone 2.4G2 is a well-characterized, literature-cited blocking antibody, making it reliable for immunology, oncology, and inflammation research.

Typical Applications

  • Flow cytometry (FACS) Fc block before staining mouse leukocytes
  • Blocking FcγRII/III in in vitro functional assays (e.g. phagocytosis, ADCC-like assays, cytokine production)
  • Pre-treatment in in vivo experiments involving therapeutic or depleting antibodies in mouse models
  • Reduction of background in staining of macrophages, dendritic cells, neutrophils, monocytes, and other Fc receptor–expressing cells