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TPO (Thyroid Peroxidase) Antibody (Thyroid Marker), Biotin Conjugate – Clone TPO/3813R – 0.1 mg/mL

The TPO (Thyroid Peroxidase) Antibody – Clone TPO/3813R is a biotin-conjugated monoclonal antibody specific for thyroid peroxidase (TPO), a key enzyme in thyroid hormone synthesis and an important thyroid autoantigen. TPO is a 933-amino acid, type I transmembrane glycoprotein expressed as a dimer on the apical surface of thyroid follicular cells. This antibody is suitable as a thyroid marker and may assist in the differentiation between benign and malignant thyroid lesions.

Biological Background

  • Thyroid hormone synthesis:
    TPO catalyzes the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester bonds between iodinated tyrosines, generating the thyroid hormones thyroxine (T4) and triiodothyronine (T3).
  • Clinical relevance:
    Mutations or anomalies in the TPO gene are associated with:
    • Congenital hypothyroidism
    • Congenital goiter
    • Thyroid hormone organification defect IIA
    Malignant thyroid tumors often exhibit altered TPO with reduced affinity for anti-TPO antibodies, making TPO immunostaining potentially useful in distinguishing benign vs. malignant thyroid tumors.

Product Features

  • Target: Thyroid Peroxidase (TPO) – Thyroid marker
  • Clone: TPO/3813R
  • Conjugate: Biotin
  • Concentration: 0.1 mg/mL
  • Key advantages:
    • Specific for TPO on thyroid follicular cells
    • Useful in thyroid pathology and tumor differentiation
    • Biotin conjugation enables flexible detection via streptavidin or anti-biotin systems in IHC, ELISA, and other assays

Format & Label Options

Primary antibodies in this series are available:

  • Purified (unconjugated)
  • Conjugated to a range of fluorescent CF® Dyes and other labels

CF® Dyes are known for:

  • High brightness
  • Excellent photostability

Note: Conjugates of blue fluorescent dyes (e.g., CF®405S, CF®405M) are not recommended for detecting low-abundance targets, as blue dyes typically have lower fluorescence and can produce higher non-specific background compared with longer-wavelength dyes.

Typical Applications

  • Immunohistochemistry (IHC) on thyroid tissue
  • Thyroid tumor profiling (benign vs. malignant marker support)
  • Studies of thyroid hormonogenesis and autoimmunity
  • Research on congenital hypothyroidism and thyroid organification defects