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The Human CD16 Antibody – Clone 3G8

The Human CD16 Antibody – Clone 3G8 is a well-characterized monoclonal antibody that recognizes human CD16 (FcγRIII), a low-affinity Fc receptor expressed primarily on NK cells, monocytes, macrophages, and some T-cell subsets. This antibody is widely used in flow cytometry, immunophenotyping, ADCC studies and immune monitoring to identify and characterize CD16-positive cell populations.

Key Features

  • Specific for human CD16 (FcγRIII)
    Recognizes CD16 expressed on NK cells, monocytes/macrophages, neutrophils and some T lymphocytes, enabling robust immune cell phenotyping.
  • Widely used clone 3G8
    Clone 3G8 is a reference clone in the literature and routine immunology workflows, ensuring high reproducibility and comparability across studies.
  • Ideal for flow cytometry
    Commonly used for FACS-based immunophenotyping, NK cell gating (e.g. CD3⁻ CD56⁺ CD16⁺) and monitoring of Fc receptor–expressing cells.
  • Suitable for functional studies
    Can be used in functional assays, including antibody-dependent cellular cytotoxicity (ADCC) and Fc receptor blocking or engagement experiments (depending on isotype and formulation).

Typical Applications

  • Immunophenotyping of NK cells, monocytes, macrophages, neutrophils
  • Flow cytometry panels for innate and adaptive immunity
  • ADCC and Fc receptor function assays
  • Immune monitoring in oncology, infectious disease and immunotherapy research