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Immunoglobulin Alpha (IgA) Heavy Chain Antibody – Clone rHISA43, Biotin Conjugate – 0.1 mg/mL

The Immunoglobulin Alpha (IgA) Heavy Chain Antibody (rHISA43) is a monoclonal antibody specific for the heavy chain of human IgA, with minimal cross-reactivity to the heavy chains of other immunoglobulin classes. It recognizes all subclasses of the IgA alpha heavy chain, making it a powerful tool for detecting IgA in serum, tissue, and mucosal samples. This format is biotin-conjugated at a concentration of 0.1 mg/mL, ideal for use in ELISA, IHC, and other biotin–streptavidin based assays.

Biological Background

Immunoglobulins are Y-shaped, four-chain monomers composed of two identical heavy chains and two identical light chains linked by disulfide bonds. They form:

  • Fab region: Antigen-binding fragment
  • Fc region: Constant fragment mediating effector functions

Immunoglobulin A (IgA):

  • Main immunoglobulin of the mucosal immune system
  • Produced predominantly in gut-associated lymphoid tissue (GALT)
  • Daily IgA production exceeds that of all other isotypes
  • Exists primarily as dimers, but also as polymers and monomers
  • Dimers/polymers contain a J chain, enabling binding to polymeric immunoglobulin receptor (pIgR) for transcytosis to mucosal surfaces

In hematopathology, plasmacytomas and some non-Hodgkin lymphomas often display restricted expression of a single heavy chain class. Demonstrating clonality of immunoglobulin expression in lymphoid infiltrates helps distinguish reactive vs. malignant processes.

Product Features

  • Target: Immunoglobulin Alpha (IgA) heavy chain
  • Clone: rHISA43
  • Specificity:
    • Specific for IgA heavy chain
    • Minimal cross-reactivity with heavy chains of other immunoglobulin classes
    • Reactive with all subclasses of α (alpha) heavy chain
  • Conjugate: Biotin
  • Concentration: 0.1 mg/mL
  • Key advantages:
    • Ideal for detection and quantification of IgA in biological samples
    • Useful in mucosal immunology, gut immunity, and B-cell / plasma cell studies
    • Supports clonality assessment in lymphoid infiltrates and IgA-related neoplasms

Format & Label Options (Family Info)

Primary antibodies in this series are available:

  • Purified (unconjugated)
  • Conjugated to a range of fluorescent CF® Dyes and other labels

CF® Dyes provide:

  • High brightness
  • Excellent photostability

Note: Conjugates with blue fluorescent dyes such as CF®405S and CF®405M are not recommended for low-abundance targets, as blue dyes typically have lower fluorescence and may yield higher non-specific background compared to longer-wavelength dyes.

Typical Applications

  • Detection of IgA-expressing plasma cells in tissue sections
  • IHC / ICC in mucosal tissues (e.g. gut, respiratory tract)
  • ELISA and immunoassays for quantification of IgA
  • Studies of mucosal immunity and gut-associated lymphoid tissue
  • Assessment of clonality in plasmacytomas and IgA-restricted lymphomas